GenicBio
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Antibody Development
Polyclonal Antibody Services  
GenicBio offers a full range of services for both polyclonal and monoclonal antibody development and production. Our custom antibodies can be generated against customer-supplied antigens or against peptides synthesized and conjugated by us. Our scientists have the knowledge and experience in antigen design, antibody production, purification and isolation, etc., which allows us to produce high-affinity, titer guaranteed and customer-satisfied antibodies.

Cat.No.

Polyclonal Antibody Production Packages

Guarantees and Deliverables

GPAB01

Anti-protein pAb package: $420.00

Antigen: Protein, 3-5mg with >85% (provided by customer)

Animal: 2 rabbits or 5 mice

Delivery time:: 9~10 weeks

Service Contents: Immunization & Anti-Sera Production, ELISA identification

Guarantees: ELISA >1:200,000

Deliverables:

Pre-immune serum: 2ml/rabbit

Original sera volume: 40~60ml from each rabbit

Titer (ELISA) result and product report

GPAB02

Customer supplied antigen standard package: $500.00

Antigen: Peptide, 5-10mg with >80% (provided by customer)

Animal: 2 rabbits or 5 mice

Delivery time:: 9~10 weeks

Service Contents: Conjugation, Immunization & Anti-Sera Production, ELISA identification

Guarantees: ELISA >1:100,000

Deliverables:

Pre-immune serum: 2ml/rabbit

Original sera volume: 40~60ml from each rabbit

Titer (ELISA) result and product report

GPAB03

Complete anti-peptide pAb package: $600.00

Antigen: Peptide, GenicBio to synthesize (up to 15aa, 20mg, >75% purity)

Animal: 2 rabbits or 5 mice

Delivery time:: 11~12 weeks

Service Contents: Peptide design & peptide synthesis, conjugation, Immunization & Anti-Sera Production, ELISA identification

Guarantees: ELISA >1:100,000

Deliverables:

10mg unconjugated peptide

Pre-immune serum: 2ml/rabbit

Original sera volume: 40~60ml from each rabbit

Titer (ELISA) result and product report

GPAB04

Phospho-specific pAb package: USD1,500.00

Antigen: Phospho peptide, GenicBio to synthesize(purity >90%)

Animal: 2 rabbits or 5 mice

Delivery time:: 12~14 weeks

Service Contents: Peptide design & peptide synthesis, conjugation, Immunization & Anti-Sera Production, antigen affinity purification, ELISA identification

Guarantees: ELISA >1:100,000, 10 × greater than non-phosphorylated peptide

Deliverables:

10mg of phospho & non-phospho peptides

Pre-immune serum: 2ml/rabbit

Affinity purified anti-phospho-peptide specific antibody that only reacts with phosphorylated site

GPAB05

Site specific pAb package - email us for a quote

Antigen: methylated or acetylated peptide, GenicBio to synthesize(purity >90%)

Animal: 2 rabbits or 5 mice

Delivery time:: 12~14 weeks

Service Contents: Peptide design & peptide synthesis, conjugation, Immunization & Anti-Sera Production, antigen affinity purification, ELISA identification

Guarantees: ELISA >1:100,000, 10 × greater than non-methylated(or acetylated) peptide

Deliverables:

10mg of methylated & non-methylated peptides

Pre-immune serum: 2ml/rabbit

Affinity purified anti-phospho-peptide specific antibody that only reacts with methylated site


Optional: 

• Protein A/G affinity purification: USD130.00
• Peptide affinity purification: USD280.00
• Animal maintenance: USD1.00 per animal per day. Extra bleed: $20.00 per collection per animal.

Monoclonal Antibody Services
• Antigens (recombinant proteins or peptides) provided by customers or produced at GenicBio
• Various packages to meet different needs
• Free antigen design
• ELISA titers are guaranteed to reach >10,000
• Phosphor-specific and other modification-specific antibodies

GMAB01: Anti-protein mAb package - $2,700.00(4~5 months)
Antigen: purified protein, at least 3mg with >90% (provided by customer, lyophilized powder).The development protocol is same as GMAB02 (without peptide synthesis and conjugation)

GMAB02: Complete anti-peptide mAb package - $2,950.00(5~6 months)
1. Peptide Synthesis and Conjugation
- Peptide design: Free of charge
- Peptide synthesis: (up to 15aa, 20mg and purity>90%); A Cys will be added to either end of the peptide for conjugation
- Conjugation: KLH, BSA, OVA or others
2. Immunization and ELISA Screening
- The conjugated peptide is used to immunize five Balb/c mice (4-5 injections)
- Tail bleeds are screened by ELISA (WB、IHC、IF and other screening methods are optional)
3. Fusion and screening
- Perform splenocyte fusion on the mice that shows best response to the antigen
- The hybridoma cells are expanded and screened by ELISA  (WB、IHC、IF and other screening methods are optional)
4. Subcloning and Ascites Production
- Subclone two (up to five) positive parental clones by limiting dilution and isotyped. 
- Two positive clones are Cryopreserved and used to produce ascites.
5. Delivery and report
- 10mg un-conjugated peptide
- 1 vial of cell culture for each hybridomas cells
- 5 ml of ascites produced from each of the hybridomas cells with ELISA titer> 1:5,000

GMAB03: Phospho-specific mAb package(5~6 months)
Please email us your requirement for a detail quote.